Salam Ahad,
This says "Amal Assadullah Asfahani" or work of Assadullah of Asfahan. This sword is 17th to 19th century most likely so no, it did not belong to Imam Ali (RA)
The name Assadullah is used to this day especially in Iran, Pakistan and Afghanistan.
You have a nice sword, but you are aiming too far in the past with its attribution. I would also point that the inscription is not an authentic Assadullah inscription, its crude so likely made later on the blade. Its still a very good sword.
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